Atelier Iris: Eternal Mana is a Turn-Based RPG and with alchemy gameplay and story components. It is the 6th core Atelier game and the first game in the Iris series, being the first Ateleir game to be released internationally. The game follows the young alchemist Klein Kiesling, who is travelling to learn more about alchemy together with his friend, the Wood Mana, Popo. One day, he is saved from a monster by Lita Blanchimont, a young Galgazit, monster hunter. Lita eventually recruits him to become an Galgazit as well, and they start working together in the town of Kavoc. However, Klein is soon caught in many troubles, and eventually becomes involved in a quest of saving both the world and Lita.

"Regallzine. A world sustained by the Great Spirit Mana and its elemental powers. Every substance and object in this world is formed by the elements. People lived under the protection and guidance of the Mana. To the people of Regallzine Mana is exist as part of nature like the wind and trees. However, there are a few select people capable of creating something out of nothing using the powers of Mana called alchemist. Klein Kiesling age 17, grandson of Dafune a famous alchemist, embarks on a journey with Popo the Mana of wood, after his grandmother´s death, to surpass her as an alchemist. Walking through the dark forest of South Esvior, Klein encounters a vicious monster. Klein bravely battles the monster, but his Mana strength slowly diminishes. When everything seemed lost, a beautiful young girl named Lita came to Klein´s rescue. Lita is a monster hunter at the neighboring town of Kavok. She advises Klein that the path to Kavok is dangerous and he will need her help. Klein rejects her advice from his selfish pride, insisting that he will make it through the forest alone. After the two depart, Klein felt ashamed for not thanking Lita for her life saving help. Exiting out from the forest, Klein stood on a cliff overlooking the great plains of Esvior. What he saw from the cliff was a city built upon a steep mountain. The city Klein saw is the infamous city to the Esviore region, known as the city in the sky, Avenberry. Klein and Lita do not know yet, that the two will knock on the gates of heaven, opening the ancient path to the legendary 'city in the sky'."