Lara Croft and the Temple of Osiris is the sequel to Lara Croft and the Guardian of Light, and the second game in a spin-off from the main Tomb Raider series. The two-player cooperative campaign from the first game is expanded to support up to four players. They need to work together in a platform environment to explore the temple, defeat hordes of enemies from the Egyptian underworld, solve puzzles, and avoid traps, using a high-angle perspective like the previous game. When playing together, the level lay-outs change to support cooperative actions.

In Lara Croft's new journey she travels to Egypt and there she joins with the rival treasure hunter Carter Bell and the imprisoned gods Horus and Isis. As a team they are have to defeat the evil god Set.